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. 2017 Aug 29;68(17):4765–4774. doi: 10.1093/jxb/erx307

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Co-immunoprecipitation (IP) assay to verify the interaction between BaMV replication protein and NbPCaP1L. Total proteins (input) were extracted from N. benthamiana leaves co-infiltrated with Agrobacteria containing the infectious BaMV/Rep-HA and OFP vector or NbPCaP1L-OFP. Equal amounts of input proteins were used for immunoblotting (IB) with antibodies against HA, OFP, and BaMV CP as indicated. rbcL, RuBisCo large subunit is used as the loading control. After IP with anti-HA antibody, the co-IP of Rep-HA, NbPCaP1L-OFP, and CP was detected using corresponding antibodies for IB.