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. 2017 May 26;68(11):2833–2847. doi: 10.1093/jxb/erx139

Fig. 8.

Fig. 8.

Interaction between ORRM5 and other editing factors by Y2H and BiFC assays. ORRM5-GFPC has been used in all the panels shown. (A) ORRM5 interacts with ORRM2, ORRM3, and ORRM4 in BiFC assays. (B) No interactions were observed when ORRM5 was co-expressed with RIP1 or itself. Scale bar (white line) represents 10 µm. (C) ORRM5 interacts with ORRM3 and ORRM4 in the Y2H assay. (D) ORRM5 does not interact with RIP1 or itself in Y2H assays. 10^6: cells were diluted to 1 × 106 cells ml−1 before spotted on a dropout media plate; 10^5: cells were diluted to 1 × 105 cells ml−1 before spotted on a dropout media plate.