Fig. 5.

Accumulation of pigments undergoes physiological acclimation independent of pyrenoid phenotype. Chl expression on a per cell basis (A) is shown as a scatterplot of individual measurements. Starting with the accumulation of carotenoids relative to Chl (B), data are plotted against growth light intensity and shown as the mean of three biological replicates ±SE. The functional PSI/PSII ratio (C) and the Chl a/b ratio (D), respectively, enable quantification of Chl allocation to PSII from spectroscopic data (E) and Chl extraction data (F). Fluorescence saturation kinetics in the presence of DCMU (t_2/3) provide a proxy for functional PSII antenna size (G). Growth rates (H) are shown as percentage change in Chl ml⁻¹ over 24 h as mean ±SE based on four measurements over the course of 6 d of three biological replicates per condition grown in quasi-continuous culture; three asterisks signify statistical significance at the level of P<0.01 estimated through ANOVA.