Fig. 7.

Effect of OsMYB1 mutation on the expression of genes involved in Pi transport and signaling. (A) RT-qPCR analysis of PHT1 genes in myb1 mutants and WT plants. (B) RT-qPCR analysis of a subset of genes involved Pi signaling in myb1 mutant and WT plants. Seeds of the mutant lines and WT plants were germinated in deionized H₂O and supplied with (HP, 200 µM Pi) or without (NP, 0 µM Pi) Pi. The rice housekeeping gene OsActin1 (LOC_Os03g50885) was used as an internal control. Values are presented as the means±SD of three biological replicates. Different letters indicate significant differences within a treatment (P<0.05, Duncan’s test).