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. 2017 Nov 30;8(1):e00841. doi: 10.1002/brb3.841

Figure 1.

Figure 1

Diagram summarizing the experimental design. White areas represent the experimental periods (T1 and T2) defined by the postnatal days (PND). The first experiment (T1) encompassed the maternal separation protocol whereby the mothers were separated from their offspring for 6 hours daily (group MS). Animals from the control group (C) remained with their mothers. Experiment 2 (T2) consisted of the free‐choice model, in which the mice had to choose between water and 0.5% sucrose solution (C+SUC and MS+SUC) or between water and 10% ethanol solution (C+EtOH and MS+EtOH). At the end of T1 and T2 blood corticosterone levels were determined and the mice subjected to the light/dark box behavioral test. All experimental groups were composed of seven animals (n = 7)