Table 2.
TaqMan probe and primer sequences used for the expression analysis
| Gene name | Probe/Primer | Sequence | 5′ | 3′ |
|---|---|---|---|---|
| Pomc | Probe | CAGGCCCGGATGCAAGCCAGCA | FAM | TAMRA |
| Forward primer | CATAGACGTGTGGAGCTGGTG | |||
| Reverse primer | CCGCCGAGAGGTCGAGTC | |||
| Cart | Probe | CCGCCTTGGCAGCTCCTTCTCATGG | FAM | TAMRA |
| Forward primer | ATCTACTCTGCCGTGGATGATG | |||
| Reverse primer | GCGCTTCAATCTGCAACACA | |||
| Npy | Probe | CCCAGAACAAGGCTTGAAGACCCTTCCATG | FAM | TAMRA |
| Forward primer | GAGACACTGATTTCAGATCTCTTAATGAG | |||
| Reverse primer | GTCAGGAGAGCAAGTTTCATTTCC | |||
| Agrp | Probe | CGGTTCTGTGGATCTAGCACCTCTGCC | FAM | TAMRA |
| Forward primer | GCAGCAGACCGAGCAGAAG | |||
| Reverse primer | CACAGCGACGCGGAGAAC | |||
| Hcrt | Probe | CCTTCCTTCTACAAAGGTTCCCTGGGCC | FAM | TAMRA |
| Forward primer | GGATTGCCTCTCCCTGAGC | |||
| Reverse primer | AGCAGCAGCAGCGTCAC | |||
| Pmch | Probe | ACAAGACCACAAAGAACACAGGCTCCAAG | FAM | TAMRA |
| Forward primer | AAAATGATGAGAGCGGCTTCATG | |||
| Reverse primer | GCAGACCGTGAGTTACGAGATT | |||
| Crh | Probe | CGCTCTCTTCTCCTCCCTTGGCAGGT | FAM | TAMRA |
| Forward primer | ACCTCGCAGAACAACAGTGC | |||
| Reverse primer | CCGCAGCCGCATGTTTAGG | |||
| Oxt | Probe | CGGATGGCTGCCGCACCGACC | FAM | TAMRA |
| Forward primer | CGCGGGCATCTGCTGTAG | |||
| Reverse primer | GCCCTAAAGGTATCATCACAAAGC | |||
| Avp | Probe | CCACATCCGACATGGAGCTGAGACAGTGT | FAM | TAMRA |
| Forward primer | CTACTTCCAGAACTGCCCAAGAG | |||
| Reverse primer | GAAGCAGCGCCCTTTGCC |