Figure 6.

Proteolysis of CidC. SDS–PAGE showing the CidC cleavage products after incubation with trypsin for 30 min at pH 6 (lanes 1–4) and pH 7 (lanes 6–9). Before proteolysis, CidC was incubated with (lanes 1 and 6) pyruvate, TPP, and Mg²⁺, (lanes 2 and 7) TPP and Mg²⁺, (lanes 3 and 8) pyruvate, and (lanes 4 and 9) Mg²⁺. CidC products are shown at the left with CidC¹ and CidC² being several kilodaltons and ∼20 kDa, respectively, smaller than CidC, while CidC^p indicates small peptides. Several bands from the marker (lane M) are labeled at the right.