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. 2017 Oct 12;9(5):1692–1705. doi: 10.1016/j.stemcr.2017.09.012

Figure 3.

Figure 3

Obox1 Mitigates Cell Hyper-proliferation and Functions at the Early Stage of Reprogramming

(A) qRT-PCR analysis shows pluripotent gene expression in reprogrammable cells on day 14 post induction with or without Obox1 or Obox1-infected MEFs on day 4, and the empty vector-infected cells as control for each group.

(B) Proliferation curves of reprogrammable cells transduced with or without Obox1.

(C) Kinetic changes of percentage of THY1+ population at indicated time points during reprogramming by FACS analysis.

(D) Percentage of kinetic changes of percentage of THY1−/SSEA1+ population during reprogramming by FACS analysis.

(E) Strategy of time-course study of Obox1 during reprogramming.

(F) Reprogrammable cells were infected with Obox1 at indicated time points and the number of Oct4-GFP+ colonies were counted at 21 days post induction.

(G) Reprogrammable cells were infected with Obox1 at indicated time points and Oct4-GFP+ cells were analyzed by FACS at 21 days post induction.

Data are presented as the mean ± SEM (n = 3); ∗p < 0.05, ∗∗p < 0.01 by Student's t test for comparison and empty vector at indicated time points as control.