Figure 6.

Obox1 Mitigates Cell Hyper-proliferation and Enlarges the Proportion of THY1⁻ Cells during Reprogramming

(A) qRT-PCR analysis of cell-cycle-related gene expression in reprogrammable cells transduced with OSKM or OSKM plus Obox1 on day 2 post induction. The expression level was normalized to Hprt.

(B) Proliferation curves of MEFs transduced with or without Obox1 or OSKM, respectively (empty vector as control).

(C) Cell-cycle analysis by FACS in MEFs or the reprogramming cells on day 2 post infection and comparison of the indicated cell-cycle phase (empty vector versus MEF; Obox1 versus empty vector).

(D) Proliferation curve of THY1⁺ and THY1⁻ cells sorted from reprogrammable cells on day 2 post induction. The cells were seeded in 24-well plates at a density of 1.2 × 10⁴ cells per well and counted at indicated time points, and the THY1⁺ population and THY1⁻ population compared in the same group.

(E) qRT-PCR analysis of cell-cycle-related gene expression in THY1⁺ and THY1⁻ cell populations sorted from reprogrammable cells on day 2 post induction. The expression levels were normalized to Hprt. THY1⁺ in vector or Obox1 group as control.

Data are presented as the mean ± SEM (n = 3); ^∗p < 0.05, ^∗∗p < 0.01 by Student's t test for comparison. See also Figure S5 and Table S4.