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. 2015 Dec 31;53(2):401–408. doi: 10.1093/jme/tjv235

Table 1.

Primer sequences used for Wolbachia w Noto PCR ( wsp , 28 S, and 16S), DENV-2 NS5 gene cDNA synthesis and qPCR, and 16S probe sequences for FISH of w Noto

Target Gene Primer/Probe Sequence (5′-3′)
wsp 81F: TGG TCC AAT AAG TGA TGA AGA AAC
691R: AAA AAT TAA ACG CTA ACT CCA
28S FWD: TAC CGT GAG GGA AAG TTG AAA
REV: AGA CTC CTT GGT CCG TGT TT
16S FWD: CAT ACC TAT TCG AAG GGA TAG
REV: AGC TTC GAG TGA AAC CAA TTC
DENV-2 NS5 FWD: ACA AGT CGA ACA ACCTGG TCC AT
REV: GCC GCA CCA TTG GTC TTC TC
16S probes W2: CTT CTG TGA GTA CCG TCA TTA TC
W3: AAC CGA CCC TAT CCC TTC GAA TA