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Fluorescent microscopy images of HEK 293 cells transfected by bolaplexes at the optimal N/P ratio (with the vector name and N/P ratio in each image). The molar ratio of bolaamphiphile/DOPE was 1:1, lipofectamine 2000 was used for comparison. The cells were observed by fluorescence microscopy after a 24 h transfection. Scale bar: 100 μm.
