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. 2017 Jul 11;68(15):4137–4151. doi: 10.1093/jxb/erx217

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Effect of seed removal and hormone treatment on the relative transcript abundance of the ethylene biosynthesis genes PsACS1 (A), PsACS2 (B), PsACS4 (C), PsACO1 (D), PsACO2 (E), and PsACO3 (F) in the pericarps of pollinated pea ovaries. Pericarps at 2 DAA were either left intact, split (SP), or split and deseeded (SPNS), and treated 12 h after deseeding with 4-Cl-IAA, IAA, or ethephon alone or in combination. When fruits were treated with both auxin and ethephon, ethephon was applied 90 min after the auxin treatment and samples were collected based on the time after auxin treatment. Because of the delayed ethephon application, the auxin- plus ethephon-treated pericarps were not studied at the 2 h time point. Deseeded pericarps were also pre-treated with STS at pericarp splitting and deseeding (STS treatment), and IAA was applied to STS-pre-treated pericarps (IAA plus STS treatment). The SP and SPNS controls were treated with 0.1% aqueous Tween-80. All the samples were collected with respect to the time after hormone treatment. Data are means ±SE, n=3–8, with the exception of STS+IAA 2 h treatment, where n=2.