Fig. 5.
Chloroplast movement and ΦII responses under fluctuating light. Three-week-old Arabidopsis plants of the indicated genotypes were subjected to the same white light regime shown for Day 3 in Fig. 4. (A) Chloroplast movement measured by reflectance in large- (upper panel) and intermediate- (lower panel) chloroplast mutants. Reflectance of pulsed red light was imaged from whole plants at each light intensity. The open and filled symbols correspond to alternating periods of ambient and fluctuating light, respectively. (B) Heat map comparing ΦII responses in Col-0 (WT) and chloroplast division mutants. The light regime is illustrated at the top. Thick and thin bars represent ambient and fluctuating light intensities, respectively. The color scale at the bottom of each heat map depicts the log-fold increase (red) or decrease (blue) in values in the mutants normalized against values in the WT (black) at each time point. For all data points, n=4–6. The error bars in (A) represent the SD. Raw data and statistical analysis for the heat map (B) are shown in Supplementary Figs S12 and S14.