Fig. 6.

Effect of Si on the expression of OsHMA2, OsHMA3, and OsNramp5 in roots of lsi1, lsi2, and their wild types (WT1 for lsi1 and WT2 for lsi2). Seedlings (10 d old) were cultivated in a solution with or without 1 mM Si for 7 d. Before sampling for RNA extraction, the roots were exposed to 1 µM Cd for 24 h. The expression of OsHMA2 (A), OsHMA3 (B), and OsNramp5 (C) was determined by quantitative RT-PCR. HistoneH3 was used as an internal standard. Expression relative to WT (–Si) is shown. Data represent means±SD (n=4). Different lower-case letters indicate significant difference at P<0.05 by Duncan’s test.