Fig. 4.
Delayed seed development of FUS3 phospho-null mutants at control temperature. Embryo development of wild-type, fus3-3, FUS3S>A, and FUS3S>D plants grown under long days at 21 °C/18 °C. (A) Representative images showing the morphology of FUS3 phosphomutant embryos excised from 11 DAP siliques, corresponding to the bent cotyledon/mature stage in the wild type. fus3-3 embryos skip dormancy and enter vegetative growth precociously, as seen from root growth and embryo size. FUS3S>A embryo growth is delayed, as seen from the presence of early embryo stages (torpedo and walking stick). Scale bar=0.5 mm. (B) Quantification of embryo stages shown in (A). Embryos dissected from four siliques per genotype were scored for morphological stages (n=60–80). (This figure is available in colour at JXB online.)