Fig. 3.

MdSnRK1.1 interacts with MdJAZ18. (A, B) MdSnRK1.1 interacts with MdJAZ18 in Y2H assays. According to the domains of MdSnRK1.1 (KD and RD domain) and MdJAZ18 (ZIM and Jas domain), the full length or derivatives thereof were used to assess their interactions. Yeast dropout medium lacking Leu and Trp (-T/-L) was used as transformation control, and that lacking Leu, Trp, His, and Ade (-T/-L/-H/-A) acted as a screen. (C) In vitro pull-down assay to verify the interaction between MdSnRK1.1 and MdJAZ18. HIS-MdSnRK1.1 was co-incubated with GST or GST–MdJAZ18 protein and then purified using a GST purification kit. The resultant protein samples were immunoblotted with anti-HIS and anti-GST antibodies, respectively.