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. 2017 May 25;68(11):2977–2990. doi: 10.1093/jxb/erx150

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — MdJAZ18 is involved in MdSnRK1.1-induced anthocyanin and PA accumulation. (A, B) Coloration (A) and anthocyanin contents (B) of WT, 35S::MdSnRK1.1-GFP, 35S::MdSnRK1.1-GFP+35S::Flag-MdJAZ18, 35S::asMdSnRK1.1, and 35S::asMdSnRK1.1 + 35S::Flag-MdJAZ18 treated with 1% sucrose or mannitol. (C, D) PA staining (C) and contents (D) in the corresponding calli shown in (A). In (B) and (D), FW, fresh weight. (E–H) The expression levels of regulatory genes (MdMYB1 and MdMYB9) and structural genes (MdDFR and MdANR) in anthocyanin and PA biosynthetic pathways by qRT-PCR analysis in the calli shown in (A). 18S acted as the internal control. In (B) and (D–H), error bars represent the SD based on three independent replicates. Statistical significance was calculated by the LSD test with DPS software, P<0.05.