Fig. 8.
OsCV interacts with the chloroplast stromal glutamine synthetase 2 in vivo. (A) Glutamine synthetase 2 protein interacts with OsCV as identified by co-immunoprecipitation and MS. (B) BiFC design: SCFPC and VenusN are fused at the C-terminus of OsGS2 and OsCV, respectively. (C–E) BiFC analysis of in vivo interactions: OsCV–VenusN and OsGS2–SCFPC (CV+GS2), by transient expression in Nicotiana benthamiana. (C) BiFC signals obtained 2 d after infiltration. Most of the signals overlapped with chloroplasts. White arrows indicate BiFC signal outside the chloroplast. Yellow arrows indicate BiFC signal in chloroplasts. (D) BiFC signals obtained 3 d after infiltration. Signals overlapped with chloroplasts and the PVC marker (RabF2a–RFP). (E) BiFC signals obtained 4 d after infiltration. Signals overlapped with the vacuolar marker (VAMP711–RFP).