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Representative RNA blot analysis showing transgene expression in the leaf tissue of wild-type (WT) and six representative independent T2 transgenic lines. HvNAATb, barley nicotianamine aminotransferase; OsNAS1, rice nicotianamine synthase; rRNA, ribosomal RNA. Wild-type and transgenic lines were grown under nutrient-sufficient conditions, and total RNA was isolated from flag leaves at physiological maturity.
