Fig. 1.

Overexpression of ABF1F, ABF1V, or ABF1E confers different degrees of osmotic-stress sensitivity in rice. (A) Representative images of indicated genotypes subjected to drought treatment. Plants were grown under long days (LDs, 14 h light/10 h dark) for 3 weeks before water deprivation. The images were taken before the water deprivation treatment (drought 0 d), after 8 d of water deprivation (drought 8 d), and then after water had been restored for 7 d (Rec 7 d). (B) Survival rate of each genotype subjected to water deprivation treatments as described in (A). Mean values ±SD are shown. The values of the indicated genotypes were compared to that of the WT (Student’s t-tests, **P<0.01, n=3). (C) Protein expression analysis of ABF1V, ABF1E, and ABF1F in the indicated lines. The immunoblot was probed with anti-OsABF1 antibody. The relative abundance of OsABF1 in each genotype was calculated by the formula: (OsABF1/Ponceau [each genotype])/(OsABF1/Ponceau [ABF1F-1]). The relative abundance of OsABF1 in ABF1F-1 was arbitrarily set to 1. Mean values ±s.e.m. (standard error of the mean) are shown (Student’s t-tests, *P<0.05, **P<0.01, n=3).