Figure 6.

Greatest magnitude of Lassa virus–specific CD4 T-cell function was observed during the acute phase of infection. A, Ex vivo stimulation followed by flow cytometry was used to examine cytokine expression from CD4 T cells upon stimulation with viral antigen. B, SPICE plots were generated to demonstrate the fraction of Lassa-specific cells that were producing each cytokine in response to antigen stimulation at each time point. Pie wedge colors refer to the legend below, whereas pie arc colors refer to the legend to the right. Abbreviations: IFN-γ, interferon γ; IL-2, interleukin 2; TNF-α, tumor necrosis factor α.