Figure 2.

Epstein-Barr virus type 2 (EBV-2) infects T cells in vivo. Peripheral blood mononuclear cells (PBMCs) were collected from infant donors at 1, 2, and 24 months of age. Non–T-cell and T-cell fractions were isolated from the total PBMC population via magnetic columns, and DNA was subsequently isolated. Multiplex quantitative polymerase chain reaction (PCR) analysis of BALF5 in the EBV genome was performed to determine the log EBV copy number per 1 × 10⁶ cells for each cellular fraction collected at ages 12 months (A) and 24 months (B). Additionally, a multiplex reverse-transcription PCR to detect EBNA3c was performed to identify the EBV type infecting each cell fraction. Post sort purity analysis for CD3 and CD19 was performed on all non–T-cell and T-cell fractions. Representative flow plots for infants 2 and 8 are shown under the corresponding bar graphs. Abbreviation: ND, not detected.