Figure 3.

Epstein-Barr virus type 2 (EBV-2) is not detected in the T-cell compartment of adults. Peripheral blood mononuclear cells (PBMCs) were collected from mothers at the time of enrollment into the study. Non–T-cell and T-cell fractions were isolated from the total PBMC population via magnetic columns, and DNA was subsequently isolated. Multiplex quantitative polymerase chain reaction (PCR) analysis of BALF5 in the EBV genome was performed to determine the log number of EBV copies per 1 × 10⁶ cells for each cellular fraction. Additionally, a multiplex reverse-transcription PCR analysis to detect EBNA3c was performed to identify the EBV type infecting each cell fraction. Postsorting purity analysis for CD3 and CD19 was performed on all non–T-cell and T-cell fractions. Representative flow plots for infants 2 and 8 are shown under the corresponding bar graphs. Abbreviation: ND, not detected.