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. 2017 Oct 20;68(20):5653–5668. doi: 10.1093/jxb/erx371

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Endoplasmic reticulum (ER) stress-induced PCD in water-stressed roots. (A) Expression levels of BAX Inhibitor-1 (BI-1), Luminal binding protein 2 (BiP2), and Pathogenesis-related protein 1 (PR1) in roots treated with 40% PEG (–1.76 MPa) for the wildtype (WT) and lines overexpressing (35S:Pgb1) or down-regulating (Pgb1-RNAi) AtPgb1. Values are normalized to the WT at 0 h (set at 1), and are means ±SE of three biological replicates. (B) Effects of 4-phenyl butyric acid (PBA), 1-methylcyclopropene (1-MCP), ethephon (ET), and diphenyleneiodonium (DPI) on root growth. Values are expressed as percentages relative to the respective control in the absence of PEG (C, –PEG) (set at 100%), and are means ±SE of three biological replicates, each consisting of 20 roots. Different letters indicate statistically significant differences (P<0.05). When applied, PEG was added at a concentration of 40% (–1.76 MPa).