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. 2017 Oct 20;68(20):5653–5668. doi: 10.1093/jxb/erx371

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Phytoglobin effects on reactive oxygen species (ROS) during water stress. (A) Localization of ROS in the wild-type (WT) and lines overexpressing (35S:Pgb1) or down-regulating (Pgb1-RNAi) AtPgb1. Roots were grown on 40% PEG (–1.76 MPa) and stained at different time points (as indicated). Scale bars are 100 μm. The graph indicates the fluorescence signal (in pixels) within the root apical tip (1mm) at 48 h. Values are means ±SE of three biological replicates. Different letters on the graph indicate statistically significant differences (P<0.05). (B) Expression levels of the Arabidopsis respiratory burst oxidases (RBOHA–D) in roots treated with 40% PEG. Values are normalized to the WT at 0 h (set at 1), and are means ±SE of three biological replicates.