Fig. 2.

Chlorophyll content, chloroplast ultrastructure and efficiency of photosynthesis of wild-type (WT) and wlp2 plants. (A, B) Chlorophyll content in the leaves at the four-leaf stage (A) and in young panicles at the heading stage (B). (C) Chlorophyll accumulation rates at 28 °C after cultivation in continuous dark for 7 d. (D–L) Chloroplast ultrastructures in cells of the third leaf of WT (D, G, J), wlp2s (E, H, K) and wlp2w (F, I, L) at 22 °C (D–F), 28 °C (G–I), and 32 °C (J–L). (M–O) Chloroplast ultrastructures in cells of young panicles of WT (M), wlp2s (N) and wlp2w (O) grown in field conditions (summer of 2013). C, chloroplast; N, nucleus, Og, osmiophilic plastoglobuli; Ic, immature chloroplast; S, starch granule; Cw, cell wall. (P) Phenotype of the samples used to measure photosynthetic parameters at the tillering stage. (Q) Net photosynthetic rates (Pn) of leaves of WT, wlp2s and wlp2w. (R) Color representation images of maximum photochemical efficiency (Fv/Fm). The color scale at the bottom of the image depicts Fv/Fm values; black circles represent sampling points in the leaves. Bars=1 cm. (S) Fv/Fm values recorded in leaves. Data in A–C, Q, and S are shown as means±SD from three individual replicates. Asterisks indicate statistical significance between WT and mutants, as determined by Student’s t-test: *P<0.05, **P<0.01.