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. 2017 Oct 16;68(18):5147–5160. doi: 10.1093/jxb/erx332

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Map-based cloning of the WLP2 gene. (A) Location of the WLP2 locus in a 3.3 cM interval on chromosome 1. (B) The location of WLP2 was narrowed to a 206.5 kb region. (C) Prediction of 35 putative ORFs in the 206.5 kb genomic region. (D) Gene structure of the WLP2 (ORF21). Black boxes represent exons; the lines between them represent introns. (E, F) Phenotype of wild-type (WT), wlp2s, and T₁ plants of transgenic wlp2s expressing WLP2 at the seedling (E) and heading (F) stages. Bar=5 cm in E and 20 cm in F. (G, H) qRT-PCR of WLP2 (G) and western blot analysis using GFP antibody to the WLP2-GFP fusion protein (H). (I, J) Chloroplast ultrastructures (I) and chlorophyll content of WT, wlp2s, and two T₁ plants (J). Bar=5 μm in I. Data in G and J are shown as means±SD from three individual replicates.