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. 2017 Oct 16;68(18):5147–5160. doi: 10.1093/jxb/erx332

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — WLP2, OsFLN2, and OsTRXz form a TRX-FLN regulatory module in the PEP complex. (A) Interaction of WLP2, OsTRXz, and OsFLN2 in a yeast two-hybrid assay. Yeast transformants were spotted on to control medium (SD/-Leu/-Trp [-LT]) and selective medium (SD/-Leu/-Trp/-His/-Ade [-LTHA] plus X-α-gal and Aureobasidin A [AbA]). (B) A bimolecular fluorescence complementation assay showed that OsTRXz can interact with WLP2 and OsFLN2 in rice protoplast cells. Bars=5 μm. (C) In vitro pull-down assay of WLP2, OsFLN2, and OsTRXz. The fusion proteins of WLP2 and OsFLN2 with a His tag (His-WLP2, His-OsFLN2) and OsTRXz with a GST tag (GST-OsTRXz) were detected by anti-His antibody and anti-GST antibody, respectively. (D–F) Levels of mRNA of WLP2 (D), OsFLN2 (E), and OsTRXz (F) under normal (26 °C) or high (32 °C) temperature conditions. (G) Immunoblot analyses of OsTRXz in wild-type (WT), wlp2 mutants, and WLP2 overexpression line 16 (OE-16) in seedlings at the third-leaf stage maintained at 26 °C and 32 °C.