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. 2017 Oct 16;68(18):5147–5160. doi: 10.1093/jxb/erx332

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 7. — The wlp2 mutants showed heat-stress-induced death with elevated ROS accumulation. (A) Phenotype of wild-type (WT) and wlp2s plants at the four-leaf stage grown under heat stress (32 °C). (B) NBT and DAB staining in blades of 2-week-old seedlings in WT, wlp2s, and a WLP2 overexpression line (OE) grown at 32 °C. (C) Microscopic analysis of leaves of 2-week-old seedlings of WT, wlp2s, and WLP2 OE lines, and protoplast cells of wlp2s (P-wlp2s), incubated with H₂DCFDA at 32 °C. Green staining represents oxidized H₂DCFDA and red represents chlorophyll. H₂DCFDA foci co-localize with chloroplasts in P-wlp2s. Bars=50 μm in WT, wlp2s, OE; bar=2 μm in P-wlp2s. (D, E) RNA-seq analysis of wlp2s and WT grown under the heat stress (32 °C) condition. (D) GO enrichment analysis of differentially expressed genes (DEGs) between wlp2s and WT (P≤0.05). (E) Expression heat map of selected DEGs.