Table 1.
Experiment No. 2: Recovery of HCV from Pooled Filtersa
| Days of Storage | Item | Filter Pool Size | Number (%) Positive | Average RLU | Standard Deviation |
|---|---|---|---|---|---|
| 0 | Input syringe | – | 53 (88.3%) | 6758 | 5175 |
| Receptive syringe | – | 60 (100%) | 27 480 | 10 290 | |
| 1 | 2 (3.3%) | 5200 | 2674 | ||
| Filters | 3 | 2 (3.3%) | 2589 | 1576 | |
| 6 | 3 (5.0%) | 2038 | 1064 | ||
| 10 | 3 (5.0%) | 13 424 | 4816 | ||
| 1 | Input syringe | – | 29 (48.3%) | 3236 | 2336 |
| Receptive syringe | – | 60 (100%) | 14,892 | 4276 | |
| Filters | 1–10 | 0 | |||
| 3 | Input syringe | – | 9 (15%) | 1187 | 202 |
| Receptive syringe | – | 60 (100%) | 2893 | 1135 | |
| Filters | 1–10 | 0 | |||
| 7 | Input syringe | – | 0 | 2249 | 408 |
| Receptive syringe | – | 60 (100%) | |||
| Filters | 1–10 | 0 |
Abbreviations: HCV, hepatitis C virus; RLU, relative luciferase activity.
aVirus proliferation from the contents recovered from input syringes with detachable needles, receptive syringes, and filters was measured by determining the RLU from the luciferase gene inserted into the chimeric HCV. For each condition listed below, 60 replications were run, and the average RLU was calculated only for those cases in which the RLU exceeded the threshold indicative of active HCV replication.