Table 1.

List of genes associated with N metabolism and transport that were differentially expressed between the LN and HN conditions in CS/1103P and/or CS/RGM at 3 and 24 h post-treatment (hpt)

Name	Function	Contig name	CRIBI accession v1	CS/1103P		CS/RGM
				HN vs. LN		HN vs. LN
				3 hpt	24 hpt	3 hpt	24 hpt
6PGDH	6-phosphogluconate dehydrogenase	mix_LOC100241717	VIT_02s0025g00900	0.91	0.5	1.85	1.04
AMT3.1	Ammonium transporter 3.1	mix_LOC100252515	VIT_07s0031g02950	–0.97	0	–1.3	–2.31
AMT3.3	Ammonium transporter 3.3	mix_LOC100248822.2.2	VIT_08s0058g00140	–0.58	0	–1.1	–1.46
NR	Nitrate reductase	mix_LOC100264320	VIT_18s0001g03910	1.21	0	2.02	0.84
NIR1	Nitrite reductase 1	mix_contig_09056	VIT_03s0063g00370	1.24	0	2.39	1.42
		mix_contig_10888	VIT_03s0063g00370	1.37	0.66	2.5	1.45
GLT1	Glutamate synthase	mix_LOC100246868	VIT_16s0098g00290	0.69	0.84	1.48	1.32
GS2	Glutamine synthetase	mix_contig_00751	VIT_05s0020g02480	1.09	0	1.83	0.93
		mix_LOC100261413.1.2	VIT_05s0020g02480	1.05	0	1.82	0.91
		mix_LOC100261413.2.2	VIT_05s0020g02480	1.03	0	1.96	0.92
GSR	Glutamate synthase	mix_contig_00892	VIT_14s0006g00350	0.72	0.63	1.2	0.55
		mix_contig_01347	VIT_17s0000g01910	0.69	0.58	1.06	0
		mix_contig_02263	VIT_14s0006g00350	0.72	0.64	1.21	0.57
		mix_contig_02304	VIT_14s0006g00350	0.7	0	1.28	0.6
		mix_contig_06858	VIT_14s0006g00350	0.7	0.6	1.14	0
		mix_GLNA2.1.7	VIT_14s0006g00350	0.84	0	1.4	0
		mix_GLNA2.2.7	VIT_14s0006g00350	0.69	0.59	1.26	0.63
		mix_GLNA2.3.7	VIT_14s0006g00350	0.69	0.61	1.3	0.6
		mix_GLNA2.4.7	VIT_14s0006g00350	0.72	0.61	1.2	0
		mix_GLNA2.5.7	VIT_15s0024g01530	0.69	0.57	1.17	0.56
		mix_GLNA2.6.7	VIT_14s0006g00350	0.78	0.64	1.16	0
		mix_GLNA2.7.7	VIT_14s0006g00350	0.74	0.61	1.26	0.58
LBD39	LOB domain-containing protein 39	mix_LOC100261250.1.3	VIT_07s0129g00330	0.94	0.64	1.51	1.05
		mix_LOC100261250.2.3	VIT_07s0129g00330	0.62	0	1.13	0.65
		mix_LOC100261250.3.3	VIT_07s0129g00330	0.74	0	1.27	0.79
NPF2.13	NRT1/PTR FAMILY 2.13	mix_LOC100250071	VIT_01s0026g01490	–0.58	0	–1.77	–3.03
NPF3.1	NRT1/PTR FAMILY 3.1	mix_LOC100250961	VIT_01s0011g03400	0	0	–1.07	0
NPF4.5	NRT1/PTR FAMILY 4.5	mix_contig_05016	VIT_18s0001g11280	0	–0.54	0	–1.39
NPF6.3	NRT1/PTR FAMILY 6.3	mix_contig_00176	VIT_02s0154g00260	0.63	0.78	1.05	1.45
		mix_contig_09300	VIT_02s0154g00260	0.84	0	1.41	1.51
NRT2.4a	Nitrate transporter 2.4a	mix_contig_00726	VIT_06s0061g00320	1.29	0.97	2.23	0.95
		mix_contig_09409	VIT_06s0061g00320	1.34	0.95	2.28	1
		mix_LOC100241340	VIT_06s0061g00320	1.31	0.95	2.25	0.93
NRT2.4b	Nitrate transporter 2.4b	mix_LOC100263699.1.2	VIT_08s0040g01500	1.4	0.87	2.27	0.89
		mix_LOC100263699.2.2	VIT_08s0040g01500	1.66	0	2.18	0
NRT2.5	Nitrate transporter 2.5	mix_LOC100260250	VIT_01s0127g00070	0.5	–0.51	1.08	–0.69
NRT3.1	Nitrate transporter 3.1	mix_contig_00610	VIT_17s0000g09470	1.32	0.88	1.97	1.02
		mix_LOC100258771.1.2	VIT_17s0000g09470	1.12	0.75	1.8	0.99
		mix_LOC100258771.2.2	VIT_17s0000g09470	1.3	0.96	1.95	1.04
		mix_NAR21	VIT_17s0000g09470	1.33	0.94	1.94	1.03
UPM1	Uroporphyrin methylase 1	mix_CICLE_v10031826mg	VIT_13s0064g01470	1.46	0.71	2.35	0.92
		mix_LOC100852901.1.4	VIT_13s0064g01470	1.52	0.69	2.39	0.99
		mix_LOC100852901.2.4	VIT_13s0064g01470	1.49	0	2.36	0.92
		mix_LOC100852901.3.4	VIT_13s0064g01470	1.33	0	2.31	0.84
		mix_LOC100852901.4.4	VIT_13s0064g01470	1.54	0	2.37	0.9

The gene names have been associated to each contig according to the CRIBI annotation v1. Log Fold Change (LFC) values are indicated for each contig and condition. When the differential expression between HN and LN roots at a given time post-treatment was significant [|LFC|>1 and False Discovery Rate (FDR)<0.01], the numbers are highlighted in grey. Bold numbers indicate when genes were found to be up-regulated in the HN root side compared with LN side. Italicized numbers indicate when genes were down-regulated.