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. 2017 Jul 31;216(6):713–722. doi: 10.1093/infdis/jix369

Figure 1.

Figure 1.

Map of Plasmodium falciparum insulin-degrading enzyme homolog (PfIDEh) plasmid inserts and plots of enzyme-linked immunosorbent assay (ELISA) results with monoclonal antibody (mAb) pairs. A, Plasmid inserts were amplified from the PfIDEh sequence by indicated primer pairs. Schematic of the PfIDEh primary structure shows relative locations of the KQQDNV and DDDNTS repeats. B, Plots show signal levels obtained from horseradish peroxidase (HRP)–labeled 3B4D12, 5A2G2, and 3A3 mAbs in ELISAs of recombinant PfIDEh (rc-PfIDEh) or of PfIDEh captured by rabbit mAb AID-1-1-9 from 3D7 parasites. C, Plots show signal levels from HRP-labeled goat antimouse antibody applied to unlabeled 3B4D12, 5A2G2, and 3A3 mAbs in ELISAs of rc-PfIDEh or of PfIDEh captured by rabbit mAb AID-1-1-9 from 3D7 parasites.