Figure 7.

Enterococcus faecalis modulates the soluble and cellular host response at the wound site. Mice were wounded and infected with 10² colony forming units (CFU) or 10⁶ CFU of E faecalis, or mock infected with phosphate buffered saline. At the indicated times, wounds were processed into single cell suspensions and subjected to (A, B) cytokine analysis, shown in pg/mL. Total number of (C) neutrophils (CD45⁺ MHCII⁻ CD11b⁺ Ly6G⁺) and (D) macrophages (CD45⁺ MHCII⁻ CD11b⁺ Ly6C⁻ CD64⁺) infiltrating into and accumulating in the skin analyzed by flow cytometry. N = 2, n = 5. Error bars represent the standard error of mean. Statistical analysis was performed using Kruskal–Wallis test with Dunn’s post-hoc test. * P < .05, ** P < .01, *** P < .001, **** P < .0001. Abbreviations: CCL, chemokine ligand; CSF, colony stimulating factor; CXC, C-X-C motif chemokine; IL, interleukin; N, biological replicates; n, technical replicates; TNF, tumor necrosis factor; IFN, interferon.