Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In vitro real time monitor splicing activity induced by ISO. Luciferase activity versus time for A549-luc-intron cells (a) or A549-luc-control cells (b) treated with 10 μM or 40 μM of ISO. (c) The normalization of luciferase activity from A549-luc-intron cells to that from A549-luc-control cells treated with 10 μM or 40 μM ISO at the same period of time. (d) RT-PCR analysis of total RNA isolated from A549-luc-intron cells treated with 10 or 40 μM ISO for 0, 4 or 8 h.
