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Monitor the inhibition of cell proliferation and splicing by ISO in a reversible fashion. (a) CKK-8 assay for the A549 cells treated with 10 μM or 40 μM of ISO over time. (b) The A549 cells were treated with 40 μM of ISO for 24 h. Then the ISO was removed and the fresh medium was added for CKK-8 assay at the indicated time. (c, d) The luciferase activity from A549-luc-intron cells or A549-luc-control cells exposed to fresh medium after 24 h treatment with (c) 10 μM or (d) 40 μM of ISO.
