Fig. 2.
Bim-Sim simulation of spheres illuminated by Gaussian beam and extended source I0 integrated by multiple point sources. (a) 6 spheres with the same size but different refractive indexes. (b) 4 × 8 Extended source. (c) Transmission image of spheres illuminated by Gaussian beam with NA = 0. (d) Absorbance image, Gaussian beam, NA = 0. (e) Transmission image, 4 × 16 extended source, NA = 0.2. (f) Absorbance image, 4 × 16 extended source, NA = 0.2. (g) Transmission image, 32 × 32 extended source, NA = 0.8. (h) Absorbance image, 32 × 32 extended source, NA = 0.8.