Skip to main content
. 2018 Feb 28;2018:4093285. doi: 10.1155/2018/4093285

Figure 1.

Figure 1

CYP1A1 mRNA and protein expression levels in bovine mammary tissue and epithelial cells. (a) CYP1A1 mRNA expression in bovine primary mammary epithelial cells treated and untreated with LPS determined using RNA-Seq (n = 3). Fragments per kilobase of transcript per million fragments mapped (FPKM) in RNA-Seq result were used to indicate the gene expression level. (b) CYP1A1 mRNA expression in normal and mastitic tissues analyzed using RT-qPCR. N1–3 and M1–3 indicate different individuals. (c) CYP1A1 mRNA expression in inflammatory epithelial cells (INE) extracted from mammary glands with clinical mastitis and normal epithelial cells (NE) from slaughtered dairy cows because of fractured legs during lactation was analyzed using RT-qPCR. The numbers 1, 2, and 3 indicate different individuals. (d) CYP1A1 mRNA expression in bovine primary mammary epithelial cells (NE) and MAC-T cells treated with or without LPS was analyzed using RT-qPCR. (e) CYP1A1 protein expression in MAC-T cells treated with or without LPS was analyzed by Western blot. GAPDH was used as an internal control for all experiments. Data were expressed as the mean ± SD. ∗ p < 0.05 versus control or normal.