Fig. 2.

The small GTPase HvSar1a localized to the EHM. (A–C) Barley epidermal cell co-expressing HvSar1a–mYFP and the ER luminal marker SP–mCherry–HDEL. (D–L) Barley epidermal cells containing Bgh haustoria at 2 dai. (D–I) Cell at two focal planes, (D–F) and (G–I), co-expressing HvSar1a–mYFP and the ER marker SP–mCherry–HDEL. HvSar1a–mYFP accumulated at ERESs at the fungal entry site, and at EHM (arrow). Note that HvSar1a–mYFP remained associated with the EHM at the tip of haustorial fingers near the host cell circumference (insets in G–I). (J–L) Cell co-expressing GFP–HvRabG3b and HvSar1a–mCherry. (M) Signal quantification of GFP–HvRabG3b and HvSar1a–mCherry along the indicated path in (J, K). hb: haustorial bodies; hf: haustorial fingers; asterisk: ERES; arrowhead: ER tubules. Scale bars: 10 μm.