Table 1.

Markers used in this study

Marker/construct	Labels	Function	Reference
DiOC6	ER membrane	—	—
ER-Tracker Blue-White	ER membrane	—	—
Hexyl rhodamine B	ER membrane	—	—
SP–YFP–HDEL	ER lumen	—	Irons et al. (2003)
SP–mCherry–HDEL	ER lumen	—	Nelson et al. (2007)
HvMLO–mCherry	Plasma membrane	Powdery mildew susceptibility	Kwaaitaal et al. (2010)
HvERD2–mCherry	ER membrane	HDEL receptor	Kwaaitaal et al. (2010)
HvLTP–mCherry	Plasma membrane	Lipid transfer protein	Kwaaitaal et al. (2010)
ST–YFP	Golgi	52-amino acid signal anchor of sialyltransferase (from rat)	Brandizzi et al. (2002)
GFP–HvRabG3b	Tonoplast/cytosol	GTPase, membrane fusion	This work
HvSar1a–mCherry	ER membrane/cytosol	GTPase, vesicle budding	This work
HvSar1a–mYFP	ER membrane/cytosol	GTPase, vesicle budding	This work
HvSar1b–mCherry	ER membrane/cytosol	GTPase, vesicle budding	This work
HvSar1c–mYFP	ER membrane/cytosol	GTPase, vesicle budding	This work
HvSec12–mCherry	ER membrane/cytosol	GEF for Sar1 activation	This work
GFP and mYFP–HvRabD2a	ER/cis-Golgi/cytosol	GTPase, membrane fusion	This work
mCherry–HvRabD2a	ER/cis-Golgi/cytosol	GTPase, membrane fusion	This work
HvSar1aT34N–mYFP (GDP-locked)	ER membrane/cytosol	Inhibition of vesicle budding	This work
HvSar1aT34N–mCherry (GDP-locked)	ER membrane/cytosol	Inhibition of vesicle budding	This work
Golgi–mCherry	Golgi	α-1,2-Mannosidase I (soybean)	Nelson et al. (2007)
CFP–YFP	Cytosol	—	Bethke et al. (2009)
HvRabD2aN121I (nucleotide-free)	ER/cis-Golgi/cytosol	Inhibition of membrane fusion	This work
HvARA6–GFP	trans-Golgi network/MVB/cytosol	GTPase, MVB maturation	Inada et al. (2016)