Fig. 7.

WTG1 is required for RNA editing at petL-5 and ndhG-50 sites in plastids. (A) Loss of WTG1 disrupts editing of plastid petL-5 and ndhG-50 sites in 18-day-old seedlings as demonstrated by bulk sequencing. Asterisks indicate editable Cs. (B) Relative mRNA abundance of petL and ndhG in 18-day-old seedlings of wild-type (WT), wtg1, and wtg1 complemented with Pro35S:WTG1. Data are presented as mean±SD of triplicates. (C) Defects in editing of petL-5 and ndhG-50 sites are restored as leaves turn green. Asterisks indicate editable Cs. (D) Validation of petL-5 and ndhG-50 editing extent in WT and wtg1 mutant plants by pyrosequencing. (E) Relative mRNA abundance of petL and ndhG in WT and wtg1 plants at different growth stages. Data are presented as mean±SD of triplicates.