Fig. 3.

Loss of TGFB1 enhances in vivo adaptive immune response to Triple Negative Breast Cancer cell lines in multiple mouse strains. a In vivo analysis of C57Bl/6 mice engrafted with EO771 (2 × 10⁶) cells with KD of TGFB1. b Surviving mice from a and naïve C57Bl/6 mice were challenged at day 90 post initial tumor engraftment with 2.5 times the initial inoculum of EO771-WT cells (5 × 10⁶ cells) to assess for an anti-tumor adaptive memory response. Representative in vivo analysis of Balb/c mice engrafted with 5 × 10⁵ JC TGFβ1 KD cells (c) and 10⁴ 4T1 TGFβ1 KD cells (d). e Overexpression of TGFβ1 in JC cells results in rapid growth of tumors in immune-competent Balb/c mice. f Engraftment of 4T1 cells with double KD of TGFβ1 and CD47 in WT Balb/c mice results in the retardation of tumor growth. N = 5 mice per group. Each panel is a representative survival and growth kinetics analysis (panel insert) from one animal study and was repeated two times with equivalent results. The dotted lines and circle markers indicate cells expressing the shScramble, while solid lines and square markers represent the cells with the indicated shRNA knockdown or protein overexpression