Table 4. Medians and statistical analysis of cytokine levels after antigen specific stimulation with subtraction of baseline.
| Dunn's multiple comparisons test | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Non TB | LTBI | Active TB | Normal range | Kruskal-Wallis test | Ag-NIL NEG vs. Ag—NIL LTBI | Ag-NIL NEG vs. Ag—NIL Act. TB | Ag—NIL LTBI vs Ag.-.NIL Act.TB | ||
| Analyte | % of patients upper normal range | % of patients upper normal range | % of patients upper normal range | pg/ml | p value | p value | p value | p value | |
| Basic FGF | 0% | 0% | 0% | 1.30–55.00 | 0,3507 | ns | 0,4541 | 0,9515 | > 0,9999 |
| Eotaxin | 0% | 12% | 4% | 1.20–39.00 | 0,4187 | ns | 0,7966 | 0,6547 | > 0,9999 |
| G-CSF | 50% | 38% | 36% | <1.50 | 0,2068 | ns | 0,2764 | 0,4318 | > 0,9999 |
| GM-CSF | 0% | 4% | 0% | 0.80–122.00 | 0,8174 | ns | > 0,9999 | > 0,9999 | > 0,9999 |
| IFN-γ | 10% | 42% | 48% | 0.60–124.00 | 0,0003 | *** | 0,0004 | 0,0027 | > 0,9999 |
| IL-1β | 45% | 42% | 64% | 0.02–0.70 | 0,3585 | ns | 0,5116 | > 0,9999 | 0,9416 |
| IL-1ra | 0% | 16% | 4% | 0.20–665.00 | 0,2291 | ns | > 0,9999 | 0,2896 | 0,72 |
| IL-2 | 0% | 69% | 68% | 0.03–90.00 | < 0,0001 | **** | < 0,0001 | < 0,0001 | 0,5463 |
| IL-4 | 5% | 15% | 8% | 0.01–3.00 | 0,311 | ns | 0,6375 | > 0,9999 | 0,5554 |
| IL-5 | 0% | 8% | 4% | 0.01–7.00 | 0,0339 | * | 0,8795 | 0,3191 | 0,028 |
| IL-6 | 70% | 46% | 44% | 0.02–9.00 | 0,6 | ns | > 0,9999 | > 0,9999 | > 0,9999 |
| IL-7 | 15% | 15% | 12% | 0.01–14.00 | 0,6539 | ns | > 0,9999 | > 0,9999 | > 0,9999 |
| IL-8 | 45% | 42% | 36% | 0.08–116.00 | 0,3979 | ns | > 0,9999 | 0,5293 | > 0,9999 |
| IL-9 | 0% | 0% | 0% | 0.38–500.00 | 0,6374 | ns | > 0,9999 | > 0,9999 | > 0,9999 |
| IL-10 | 30% | 15% | 20% | 0.10–2.00 | 0,0397 | * | 0,035 | 0,2389 | > 0,9999 |
| IL-12(p70) | 15% | 15% | 16% | 0.10–6.00 | 0,0421 | ns | 0,0672 | 0,0792 | > 0,9999 |
| IL-13 | 0% | 23% | 28% | 0.01–9.00 | 0,0033 | ** | 0,0022 | 0,1727 | 0,3893 |
| IL-15 | 25% | 42% | 36% | 0.06–5.00 | 0,6884 | ns | > 0,9999 | > 0,9999 | > 0,9999 |
| IL-17 | 25% | 42% | 36% | 0.22–31.00 | 0,7224 | ns | > 0,9999 | > 0,9999 | > 0,9999 |
| IP-10 | 45% | 69% | 84% | 5.90–637.00 | 0,0026 | ** | 0,0048 | 0,0088 | > 0,9999 |
| MCP-1 (MCAF) | 45% | 81% | 68% | 2.00–48.00 | 0,4415 | ns | 0,6271 | > 0,9999 | > 0,9999 |
| MIP-1a | 40% | 38% | 32% | 0.01–2.00 | 0,3835 | ns | > 0,9999 | 0,5069 | > 0,9999 |
| MIP-1b | 50% | 31% | 60% | 1.70–47.00 | 0,0835 | ns | 0,9293 | 0,8343 | 0,0778 |
| PDGF–BB | 0% | 0% | 0% | 6.00–3,667.00 | 0,0423 | * | 0,1245 | 0,0486 | > 0,9999 |
| RANTES | 20% | 23% | 24% | 100.00–2,282.00 | 0,7542 | ns | > 0,9999 | > 0,9999 | > 0,9999 |
| TNF-α | 20% | 35% | 28% | 0.10–98.00 | 0,79 | ns | > 0,9999 | > 0,9999 | > 0,9999 |
| VEGF | 15% | 8% | 4% | 0.01–9.00 | 0,0015 | ** | 0,0034 | 0,0045 | > 0,9999 |
| CTACK | 0% | 0% | 0% | 1.00–1,086.00 | 0,5625 | ns | 0,9307 | > 0,9999 | > 0,9999 |
| GRO-a | 40% | 59% | 44% | 9.00–365.00 | 0,7153 | ns | > 0,9999 | > 0,10000 | > 0,10001 |
| HGF | 0% | 0% | 0% | 63.00–1,868.00 | 0,1554 | ns | 0,1701 | 0,981 | 0,8104 |
| IFN-α2 | 0% | 12% | 17% | 3.30–63.00 | 0,0133 | * | 0,1087 | 0,0104 | > 0,9999 |
| IL-1a | 30% | 47% | 33% | 0.40–1.40 | 0,6236 | ns | > 0,9999 | 0,9955 | > 0,9999 |
| IL-2Ra | 0% | 0% | 0% | 28.00–594.00 | 0,0195 | * | 0,0401 | 0,0275 | > 0,9999 |
| IL-3 | 0% | 47% | 67% | 13.00–170.00 | 0,0419 | * | 0,1203 | 0,0451 | > 0,9999 |
| IL-12(p40) | 0% | 24% | 22% | 36.00–646.00 | 0,0037 | ** | 0,0133 | 0,0046 | > 0,9999 |
| IL-16 | 0% | 0% | 0% | 10.00–1,270.00 | 0,3716 | ns | 0,6493 | > 0,9999 | 0,758 |
| IL-18 | 0% | 0% | 0% | 9.00–812.00 | 0,3452 | ns | 0,5321 | 0,6017 | > 0,9999 |
| LIF | 0% | 59% | 50% | 4.00–55.00 | 0,0346 | * | 0,0629 | 0,0519 | > 0,9999 |
| MCP-3 | 20% | 53% | 50% | 1.00–78.00 | 0,1776 | ns | 0,1908 | 0,6126 | > 0,9999 |
| M-CSF | 0% | 6% | 0% | 6.00–208.00 | 0,3932 | ns | 0,8376 | 0,5487 | > 0,9999 |
| MIF | 40% | 47% | 17% | 6.00–2,003.00 | 0,0029 | ** | > 0,9999 | 0,007 | 0,0208 |
| MIG | 0% | 53% | 72% | 86.00–7,911.00 | 0,0008 | *** | 0,0029 | 0,0013 | > 0,9999 |
| b-NGF | 20% | 71% | 89% | <1.10 | 0,0044 | ** | 0,0102 | 0,0081 | > 0,9999 |
| SCF | 0% | 0% | 0% | 16.00–837.00 | 0,0004 | *** | 0,0051 | 0,0004 | > 0,9999 |
| SCGF-b | 10% | 6% | 0% | 6,054.00–13,0932.00 | 0,3387 | ns | > 0,9999 | 0,4237 | > 0,9999 |
| SDF-1a | 20% | 41% | 39% | 8.00–92.00 | 0,2878 | ns | 0,68 | 0,36 | > 0,9999 |
| TNF-β | 0% | 41% | 50% | 0.71–13.00 | 0,0098 | ** | 0,0832 | 0,0077 | > 0,9999 |
| TRAIL | 0% | 6% | 17% | 8.00–272.00 | 0,0013 | ** | 0,0092 | 0,0013 | > 0,9999 |
Bold cytokines show appreciable and statistically significant differences between non-TB and LTBI or active TB
*p<0.05
**p<0.01
*** p<0.001.