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. 2018 Mar 15;13(3):e0194129. doi: 10.1371/journal.pone.0194129

Table 1. Information about the candidate reference genes.

Gene name Gene description Accession number/RNA-Seq number Primer sequence (5′–3′) Amplicon length (bp) Amplificationefficiency (%) R2
ACTIN2 Morus alba actin 1 (ACT1) HQ163776 F:GGCCATTCAAGCCGTTCTTTCTCTA R:AATTTCATCAAGTGGTCGGTGAGAT 174 105.6 0.992
ACTIN3 Morus alba actin 3 (ACT3) HQ163775 F: GAGGGCCGTGTTCCCCAGCATCGTC R: TCTTTTTGATTGAGCCTCATCCCCT 106 95.3 0.996
ACTIN4 Actin Unigene23275/ KT793030 F: TGTTGCTCCACCAGAGAGAAAGTAC R: GGACAATTGATGGACCAGACTCG 125 95.6 0.992
TUB2 Tubulin beta-3 chain CL1595.Contig6 F: GGATACCCAATAATGTGAAGTCTAGC R: CGTGAACTGCTCGCTGACCCTC 128 99.3 0.996
TUB3 Tubulin beta-1 chain protein CL8311.Contig1 F: CAAGGGTCACTACACTGAGGGAGCA R: TCGGTGATGGGAACACAGAGAATGT 215 100.4 0.994
TUB4 Tubulin alpha chain CL2672.Contig2 F: CCAAACAGACCAAGAAGAGGTAGAA R: CATGCTCAAGGCAGTAAAGCTCC 120 97.1 0.998
UBI3 Ubiquitin-like protein Unigene18627 F: ACAGGCACGAGAGCCGACAAGATT R: CCAAGCTCCACTAGCGTATAGAACA 167 101.0 0.998
UBI4 Ubiquitin-conjugating enzyme E2 Unigene5350 F: TCTCTAACCCCGAGAAATCTCTCAC R: ACGACACTCGATCCGCCTGAGC 129 101.3 0.999
UBI5 ubiquitin-activating enzyme E1 CL1942.Contig2 F: ACGGTGCATTCCTTTCCACAC R: TTCTCATTGACGTCGCATACTCAC 144 99.2 0.997
EF1α1 Elongation factor 1-alpha CL4198.Contig2 F: TAAATATAGGACAAAGCCATTTCCC R: CGACTTTCCAGAGTCAACGTGG 194 105.7 0.993
EF1α3 Elongation factor 1-alpha CL272.Contig1 F: TTGGTGTCATAAAGAGCGTTGAGAA R: ATGAAGAAGAAAATCTCGTGGCAAA 115 102.4 0.997
EF1α4 Elongation factor 1-alpha CL155.Contig3 F: ACGGTGGCGACAGGGCGTGT R: ACATCTCAACCCCAGTAACTGTGGT 109 97.5 0.997
GAPDH1 Glyceraldehyde-3-phosphate dehydrogenase CL3772.Contig5 F: ATCCCTAACATTGGCATTTCCTCG R: CCTACCGATTCTTCCAAAACCGTTG 169 99.1 0.993
GAPDH2 Glyceraldehyde-3-phosphate dehydrogenase CL131.Contig2 F: TCCAGGGTTTGAAGGACAGTGGC R: CTTCTCATTCCAAAGCGCATCATCC 138 95.7 0.996
CYP1 Cyclophilin Unigene15318 F: CTTTAGCCATTTCTCATTTTCAGTG R: GGTGGAAGGACGATCCCTTGTAG 240 98.2 0.999
MDH1 Malate dehydrogenase Unigene16243 F: GCGTCGTGGCTACTCCGTTCACT R: TCTCCTCACCCGCATATCCTTTA 215 98.1 0.999
MDH2 Malate dehydrogenase Unigene15890 F: GGAGGATGCCTTGACGGGTATGG R: GCCGCAATGGGAACAGTGGAG 198 99.7 0.990
PP2A2 Protein phosphatase 2A Unigene31643 F: TGAATCGAAGAATGAGATGTGTTCC R: AAAAATAGCAGTTTGTTTAATATGCC 138 96.6 0.997
RPL4 Ribosomal protein L4-like Unigene26991 F: GTCTCCAATGGCAGCCACAGC R: CATCGGGTAGGGCGACGGTTT 104 99.1 0.991
RPL35 Ribosomal protein L35 CL8011.Contig2 F: TCTCCGTCCCAAGAAGACCAG R: TGAAATGGAACCCACCCTACA 143 96.0 0.995