Fig. 1.
(A) A ventricular hPSC-CM AP and FP. Composing active currents are schematically depicted during time. (B) Equivalent circuit of a cell on a MEA. Ram) apical cell membrane resistance to the bath; Cam) apical membrane capacitance to the bath; Cjm) cell membrane capacitance to junction; Rjm) membrane resistance to the junction; Rb) bath resistance; Cbe) electrode capacitance via the bath resistor; Csh) shunt capacitance of the electrode; Rj) junction resistance; Cje) junction capacitance of the electrode; Ip) injection point of the AP (Vm) in the simulation circuit. (C) Measured AP of a spontaneous beating mouse E17.5 cardiomyocyte. (D) Power spectrum of panel C, peak frequency at 1 Hz. (E) Result after Chybechev IIR filtering (Fc = 30 Hz) of the AP in panel C. (F) Power spectrum of panel E, peak frequency at ∼3.5 Hz. (G) Measured FP of a mouse E17.5 cardiomyocyte on a MEA. (H) Power spectrum of panel G, peak frequency at ∼3 Hz.