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. 2018 Mar 1;544:64–71. doi: 10.1016/j.ab.2017.12.008

Fig. 1.

Fig. 1

A representation of the DNA preparation and amplification scheme. The entire block is a 420-bp section of the HIV pol gene (nt 2761 to 3180) that was purchased from Integrated DNA Technologies. The green arrows indicate the sections targeted by PCR primers for DNA standard preparation. The blue arrows indicate the sections targeted by RPA primers for the integrated assay. The RPA assay amplifies a region that contains several major drug resistance mutations, K103N, V106M, Y181C, and M184V (wild type codons denoted with red lines).