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. 2018 Mar 15;13(3):e0193954. doi: 10.1371/journal.pone.0193954

Fig 1. Schematic representation of experimental design.

Fig 1

(A) Sheep adult fibroblasts (SAFs) were isolated from ear biopsies and cultured between two and eight passages. Twenty-four hours before transfection, SAFs were cultured with 0.5% FBS and various concentrations of trichostatin A (TSA). SAFs were transfected with pEGFPC2-mPrm1, and nuclear remodeling and transfection efficiency were evaluated 48 h after transfection. (B) SAFs transfected with pEGFPC2-mPrm1 replaced somatic histone with mouse Prm1 and compacted the nucleus in a spermatid-like structure.