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. 2018 Mar 15;13(3):e0194012. doi: 10.1371/journal.pone.0194012

Fig 2. Size exclusion chromatography (SEC) fractionation and liquid chromatography-mass spectrometry (LC-MS) screening of WSC.

Fig 2

Here, (A) SEC of WSC using P6 gel (Polyacrylamide, 1–6 kDa, water as mobile phase) with UV-Vis detector at 280nm. 96 SEC fractions from A1-H12 were collected in a 96-well microtiter plate and (B) LC-MS screening of the fractions collected in SEC were performed in the same order as they were collected (A1-H12). Peak 1 to peak 5: Measured signals from LC-MS extracted ion chromatograms for abundant components profiled by LC-MS and assigned based on SEC retention times, aligned from peak No. 1 to peak No. 5 in the SEC chromatogram.