. 2018 Mar 15;13(3):e0194156. doi: 10.1371/journal.pone.0194156

Table 3. Primer information for detecting SNPs in EGLN3 coding regions.

Names	Target regions	Primers sequence (5’-3’)	Production length (bp)	Annealing temperature (°C)
E1	Exon1	F¹: `CAAGATGCTGCGTGAAGTG`	687	50.5
E1	Exon1	R²: `CTGGTATGAGGAAGGCGAAT`	687	50.5
E2	Exon2	F: `AGGCTGTCACTAGATCACTA`	702	58.4
E2	Exon2	R: `AGGCAGAGTCATCAACAAC`	702	58.4
E3	Exon3	F: `CCAGTGTTGTCATATAGC`	727	57.8
E3	Exon3	R: `ATCTGATGTTGGTAGGAG`	727	57.8
E4	Exon4	F: `CCTCATCACCATCCTGTTC`	605	53.6
E4	Exon4	R:`ACACCAGACTCATACTAAGAC`	605	53.6
E5	Exon5	F: `GGAGCAGAAGGAGAACTATT`	727	54.6
E5	Exon5	R: `CCAGCAACTTACTCTCAGAT`	727	54.6

¹ The forward sequence of the primer.

² The reverse sequence of the primer.