Table 2.
Mutations identified in resistant isolates. Strains were isolated on 5X or 10X MIC on solid medium and were confirmed as resistant by determining MICs. AN6291-RM1 had a large deletion encompassing mce3R. Mutations were identified by whole genome sequencing in five strains (indicated by an asterisk). Mutations in the remaining strains were identified by PCR amplification and sequencing of the gene.
| Compound | Strain | Gene(s) | Mutation | Amino Acid change |
|---|---|---|---|---|
| AN6288 | RM1* | ndh | 667C > A | P223T |
| AN6288 | RM2* | ndh | 1262 T > C | F421S |
| AN6291 | RM1* | Rv1948c-Rv1970 | deletion | |
| AN6291 | RM2 | mce3R | 334 T > C | W112R |
| AN6291 | RM3 | mce3R | +149–150 deletion | |
| AN6291 | RM4 | mce3R | 334 T > C | W112R |
| AN6291 | RM5* | mce3R | 563G > T | C188F |
| AN6291 | RM6* | mce3R | 555C > A | Y185* |
| AN6291 | RM7 | mce3R | +649–936 deletion | |
| AN11987 | RM1 | ndh | 994G > A | V332 M |
| AN11987 | RM2 | ndh | 994G > A | V332 M |
| AN11987 | RM3 | ndh | 998C > T | A333V |