Fig. 5.

1,25(OH)₂D₃suppresses cytokine expression by pre-committed Th17, Th17.1 and Th1 cells but most potently affects novel phenotype induction. CD4⁺ T cells from healthy control blood were stimulated with monocytes and anti-CD3 for seven days and sorted into IL-17+IFNγ- (Th17), IL-17+IFNγ+ (Th17.1), IL-17-IFNγ+ (Th1) or double negative (DN) subsets using cytokine secretion assays and flow cytometry. After two day culture with monocytes and anti-CD3 in the presence or absence of 1,25(OH)₂D₃ (+1,25D) T cells were re-analysed for IL-17 and IFNγ expression by flow cytometry. (A) Data from one donor, representative of three studied. (B) Effect of 1,25(OH)₂D₃ upon total IL-17 and total IFNγ expression by sorted subsets, calculated as the ratio of cells expressing the cytokine under untreated vs treated conditions.